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Journal: Physiological Reports
Article Title: Influence of metabolic stress and metformin on synaptic protein profile in SH‐SY5Y‐derived neurons
doi: 10.14814/phy2.15852
Figure Lengend Snippet: Signaling changes following 48 h of HI, MET, or HI/MET treatments. (a) AKT S473 phosphorylation, (b) mTOR S2448 phosphorylation, (c) p70S6K T389 phosphorylation, (d) IRS‐1 S636 phosphorylation, (e) Total IRS‐1 content, (f) total IR α content, (g) total IR β content, (h) AMPK T172 phosphorylation, (i) ULK S555 phosphorylation, (j) raptor S792 phosphorylation, as a marker of mTORC1 inhibition. Percent change is represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 and indicate differences between groups as determined using a one‐way ANOVA followed by Fischer's LSD post hoc analysis.
Article Snippet: Total IR α (Cell signaling #74118 (Dall'Agnese et al., )),
Techniques: Marker, Inhibition